Difference between revisions of "Part:BBa K5443001"
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<partinfo>BBa_K5443001 short</partinfo>
 
<partinfo>BBa_K5443001 short</partinfo>
  
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This enzyme from Saccharothrix espanaensis catalyzes the deamination of L-tyrosine to 4-coumaric acid, which is the first step in the conversion of tyrosine to vanillin. This reaction is critical as it initiates the entry of tyrosine into the phenylpropanoid pathway. Tyrosine ammonia-lyase (TAL) is a member of the aromatic amino acid lyase family, which also includes phenylalanine (PAL) and histidine ammonia-lyases (HAL). TAL is highly specific for L-tyrosine, catalysing its non-oxidative deamination, making a trans-2,3-unsaturated substituted propenoic acid and ammonia.
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This enzyme from <i>Saccharothrix espanaensis</i> catalyses the deamination of L-tyrosine to 4-coumaric acid, which is the first step in the conversion of tyrosine to vanillin (Fig. 1). This reaction is critical as it initiates the entry of tyrosine into the phenylpropanoid pathway. Tyrosine ammonia-lyase (TAL) is a member of the aromatic amino acid lyase family, which also includes phenylalanine (PAL) and histidine ammonia-lyases (HAL). TAL is highly specific for L-tyrosine, catalysing its non-oxidative deamination, making a trans-2,3-unsaturated substituted propenoic acid and ammonia.
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This part builds on the work of earlier iGEM teams, who contributed TAL Parts as follows: [https://parts.igem.org/Part:BBa_K1197011 BBa_K1197011], [https://parts.igem.org/Part:BBa_K1033000 BBa_K1033000], and [https://parts.igem.org/Part:BBa_K2997011 BBa_K2997011].
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We showed that the <i>Saccharothrix</i> TAL enzyme was functional in <i>E. coli<i> as part of a vanillin biosynthetic pathway, as evidenced by production of p-coumaric acid from tyrosine in one clone, named pMC3C-11 (see LC-MS data in the [https://parts.igem.org/Part:BBa_K5443001:Experience experience] page).
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<figure>
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    <img src="https://static.igem.wiki/teams/5443/tal-reaction.jpg" style="width:500px;height:auto">
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    <figcaption>
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        <b>Figure 1. TAL-catalysed conversion of tyrosine to p-coumaric acid.</b><br>
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        <i>This reaction represents the first step in our vanillin synthesis pathway, where the enzyme Tyrosine Ammonia Lyase (TAL) converts Tyrosine into p-Coumaric Acid.</i>
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    </figcaption>
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</figure>
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</html>
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This part builds on the work of earlier iGEM teams, who contributed TAL Parts as follows: BBa_K1197011, BBa_K1033000, BBa_K2997011. We showed that the Saccharothrix TAL enzyme was functional in E.coli as part of a vanillin biosynthetic pathway, as evidenced by production of p-coumaric acid from tyrosine (see LC-MS data from one clone pMQ3C-11).
 
  
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https://static.igem.wiki/teams/5443/tal-reaction.jpg
 
  
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https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg
 
  
 
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Revision as of 09:25, 2 October 2024


Tyrosine ammonia lyase (TAL) - (SAM8)

This enzyme from Saccharothrix espanaensis catalyses the deamination of L-tyrosine to 4-coumaric acid, which is the first step in the conversion of tyrosine to vanillin (Fig. 1). This reaction is critical as it initiates the entry of tyrosine into the phenylpropanoid pathway. Tyrosine ammonia-lyase (TAL) is a member of the aromatic amino acid lyase family, which also includes phenylalanine (PAL) and histidine ammonia-lyases (HAL). TAL is highly specific for L-tyrosine, catalysing its non-oxidative deamination, making a trans-2,3-unsaturated substituted propenoic acid and ammonia.

This part builds on the work of earlier iGEM teams, who contributed TAL Parts as follows: BBa_K1197011, BBa_K1033000, and BBa_K2997011.

We showed that the Saccharothrix TAL enzyme was functional in E. coli<i> as part of a vanillin biosynthetic pathway, as evidenced by production of p-coumaric acid from tyrosine in one clone, named pMC3C-11 (see LC-MS data in the experience page).


Figure 1. TAL-catalysed conversion of tyrosine to p-coumaric acid.
This reaction represents the first step in our vanillin synthesis pathway, where the enzyme Tyrosine Ammonia Lyase (TAL) converts Tyrosine into p-Coumaric Acid.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 484
    Illegal AgeI site found at 1095
  • 1000
    COMPATIBLE WITH RFC[1000]