Difference between revisions of "Part:BBa K5198006"
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Minimal version of the Cytomegalovirus (CMV) promoter for expression in mammalian cells. miniCMV is a shorter version of the full-length CMV promoter that retains core elements of the CMV promoter for basic transcriptional activity. | Minimal version of the Cytomegalovirus (CMV) promoter for expression in mammalian cells. miniCMV is a shorter version of the full-length CMV promoter that retains core elements of the CMV promoter for basic transcriptional activity. | ||
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| − | This variant of miniCMV was originally used as part of a Gal4UAS system (**BBa_K4839011)**, where it was placed downstream of upstream activating sequence (UAS) [1]. Upon binding of Gal4VP64 ( | + | This variant of miniCMV was originally used as part of a Gal4UAS system (**BBa_K4839011)**, where it was placed downstream of upstream activating sequence (UAS) [1]. Upon binding of Gal4VP64 (<a>href="http://parts.igem.org/Part:BBa_K3132000">BBa_K3132000</a>), the activity of miniCMV is enhanced, driving the expression of downstream genes. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 09:22, 2 October 2024
minimal CMV
Minimal version of the Cytomegalovirus (CMV) promoter for expression in mammalian cells. miniCMV is a shorter version of the full-length CMV promoter that retains core elements of the CMV promoter for basic transcriptional activity. This variant of miniCMV was originally used as part of a Gal4UAS system (**BBa_K4839011)**, where it was placed downstream of upstream activating sequence (UAS) [1]. Upon binding of Gal4VP64 (href="http://parts.igem.org/Part:BBa_K3132000">BBa_K3132000), the activity of miniCMV is enhanced, driving the expression of downstream genes. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1] L. Morsut *et al.*, “Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors,” *Cell*, vol. 164, no. 4, pp. 780–791, Feb. 2016, doi: [10.1016/j.cell.2016.01.012](https://doi.org/10.1016/j.cell.2016.01.012).