Difference between revisions of "Part:BBa K5198006"

 
Line 3: Line 3:
 
<partinfo>BBa_K5198006 short</partinfo>
 
<partinfo>BBa_K5198006 short</partinfo>
  
−
Minimal version of the Cytomegalovirus (CMV) promoter for expression in mammalian cells. The promoter can be used with upstream activating sequence in genetic designs for inducible expression.
+
Minimal version of the Cytomegalovirus (CMV) promoter for expression in mammalian cells. miniCMV is a shorter version of the full-length CMV promoter that retains core elements of the CMV promoter for basic transcriptional activity.
 +
 
 +
This variant of miniCMV was originally used as part of a Gal4UAS system (**BBa_K4839011)**, where it was placed downstream of upstream activating sequence (UAS) [1]. Upon binding of Gal4VP64 (**BBa_K3132000)**, the activity of miniCMV is enhanced, driving the expression of downstream genes.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 17: Line 19:
 
<partinfo>BBa_K5198006 parameters</partinfo>
 
<partinfo>BBa_K5198006 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
===References===
 +
 +
[1] L. Morsut *et al.*, “Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors,” *Cell*, vol. 164, no. 4, pp. 780–791, Feb. 2016, doi: [10.1016/j.cell.2016.01.012](https://doi.org/10.1016/j.cell.2016.01.012).

Revision as of 09:15, 2 October 2024


minimal CMV

Minimal version of the Cytomegalovirus (CMV) promoter for expression in mammalian cells. miniCMV is a shorter version of the full-length CMV promoter that retains core elements of the CMV promoter for basic transcriptional activity.

This variant of miniCMV was originally used as part of a Gal4UAS system (**BBa_K4839011)**, where it was placed downstream of upstream activating sequence (UAS) [1]. Upon binding of Gal4VP64 (**BBa_K3132000)**, the activity of miniCMV is enhanced, driving the expression of downstream genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] L. Morsut *et al.*, “Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors,” *Cell*, vol. 164, no. 4, pp. 780–791, Feb. 2016, doi: [10.1016/j.cell.2016.01.012](https://doi.org/10.1016/j.cell.2016.01.012).