Difference between revisions of "Part:BBa K255004"
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genome. Further the growth of the transformant was also characterized at | genome. Further the growth of the transformant was also characterized at | ||
different lactose concentrations. | different lactose concentrations. | ||
| − | [[Image: | + | [[Image:p4n.png|thumb|100px|right|alt=A |Expression of BBa_K255004 in lacI deleted E.coli strain]] |
| − | [[Image:auto.png|thumb| | + | [[Image:auto.png|thumb|100px|right|alt=A |Autofluorescence from lacI deleted E.coli host strain]] |
Revision as of 16:56, 31 October 2009
pTet.lacI-cfp(fusion).terminator.pTet.yfp.terminator
Network without any feedback and feedforward loop, present in pSB1A2 vector. BBa_K255004 represents a open loop system with constitutive expression of LacI and replication of plasmid copy. Both LacI and plasmid copy number are independent of IPTG concentration (inducer). The copy number is characterized by YFP while LacI is characterized by LacI-CFP fusion protein. YFP was measured using FACS and the distribution obtained was independent of IPTG. Beta-galactosidase was also measured at various lactose concentration to characterize the expression of gene in the host genome. Further the growth of the transformant was also characterized at different lactose concentrations.
Applications of BBa_K255004
This part was used as open loop system for our experiments.
Safety
Because this part is from commonly used elements in bacteria. No safety problem can be arised by this part.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1161
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]