Difference between revisions of "Part:BBa K255004"
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<partinfo>BBa_K255004 short</partinfo>
 
<partinfo>BBa_K255004 short</partinfo>
  
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Network without any feedback and feedforward loop, present in pSB1A2 vector.  
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Network without any feedback and feedforward loop, present in pSB1A2 vector. BBa_K255004 represents a open loop system with constitutive expression of
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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BBa_K255004 represents a open loop system with constitutive expression of
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LacI and replication of plasmid copy. Both LacI and plasmid copy number
 
LacI and replication of plasmid copy. Both LacI and plasmid copy number
 
are independent of IPTG concentration (inducer). The copy number is
 
are independent of IPTG concentration (inducer). The copy number is
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genome. Further the growth of the transformant was also characterized at
 
genome. Further the growth of the transformant was also characterized at
 
different lactose concentrations.
 
different lactose concentrations.
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[[Image:p4.png|thumb|100px|right|alt=A |Expression of BBa_K255004 in lacI deleted E.coli strain]]
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[[Image:p4.png|thumb|200px|right|alt=A |Expression of BBa_K255004 in lacI deleted E.coli strain]]
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===Applications of BBa_K255004===
 
===Applications of BBa_K255004===
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This part was used as open loop system for our experiments.
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This part was used as open loop system in our in experiments.
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===Safety===
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Because this part is from commonly used elements in bacteria. No safety problem can be arised by this part.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
  
  

Revision as of 16:44, 31 October 2009

pTet.lacI-cfp(fusion).terminator.pTet.yfp.terminator

Network without any feedback and feedforward loop, present in pSB1A2 vector. BBa_K255004 represents a open loop system with constitutive expression of LacI and replication of plasmid copy. Both LacI and plasmid copy number are independent of IPTG concentration (inducer). The copy number is characterized by YFP while LacI is characterized by LacI-CFP fusion protein. YFP was measured using FACS and the distribution obtained was independent of IPTG. Beta-galactosidase was also measured at various lactose concentration to characterize the expression of gene in the host genome. Further the growth of the transformant was also characterized at different lactose concentrations.

A
Expression of BBa_K255004 in lacI deleted E.coli strain


Applications of BBa_K255004

This part was used as open loop system in our in experiments.

Safety

Because this part is from commonly used elements in bacteria. No safety problem can be arised by this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1161
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]