Difference between revisions of "Part:BBa K255004"

Revision as of 16:38, 31 October 2009

pTet.lacI-cfp(fusion).terminator.pTet.yfp.terminator

Network without any feedback and feedforward loop, present in pSB1A2 vector.

Sequence and Features

Assembly Compatibility:

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 <partinfo>BBa_K255004 short</partinfo>
-Network without any feedback and feedforward loop, present in pSB1A2 vector. BBa_K255004 represents a open loop system with constitutive expression of
+Network without any feedback and feedforward loop, present in pSB1A2 vector.
+<!-- Add more about the biology of this part here
+===Usage and Biology===
+BBa_K255004 represents a open loop system with constitutive expression of
 LacI and replication of plasmid copy. Both LacI and plasmid copy number
 are independent of IPTG concentration (inducer). The copy number is
 characterized by YFP while LacI is characterized by LacI-CFP fusion
-protein.
+protein. YFP was measured using FACS and the distribution obtained was
+independent of IPTG. Beta-galactosidase was also measured at various
+lactose concentration to characterize the expression of gene in the host
+genome. Further the growth of the transformant was also characterized at
+different lactose concentrations.
+[[Image:p4.png|thumb|100px|right|alt=A |Expression of BBa_K255004 in lacI deleted E.coli strain]]
+===Applications of BBa_K255004===
+This part was used as open loop system for our experiments.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->