Difference between revisions of "Part:BBa K5443003:Experience"
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| + | ===Applications of BBa_K5443003=== | ||
| − | + | We showed that the HpaBC enzyme was functional in E.coli as part of a vanillin biosynthetic pathway, as evidenced by production of caffeate (see LC-MS data from one clone pMQ3C-11). Further evidence for functional expression of the HpaBC monooxygenase was seen from production of a brown melanin-type dye as a side-reaction; we believe this indicates that HpaBC is acting on tyrosine (to make L-DOPA, which polymerises) in addition to our intended reaction on 4-coumarate to make caffeate. | |
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| − | + | https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg | |
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| + | https://static.igem.wiki/teams/5443/melanin-pmq3c.jpg | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 08:45, 2 October 2024
Applications of BBa_K5443003
We showed that the HpaBC enzyme was functional in E.coli as part of a vanillin biosynthetic pathway, as evidenced by production of caffeate (see LC-MS data from one clone pMQ3C-11). Further evidence for functional expression of the HpaBC monooxygenase was seen from production of a brown melanin-type dye as a side-reaction; we believe this indicates that HpaBC is acting on tyrosine (to make L-DOPA, which polymerises) in addition to our intended reaction on 4-coumarate to make caffeate.
User Reviews
UNIQc2b042a7b4647ff1-partinfo-00000000-QINU UNIQc2b042a7b4647ff1-partinfo-00000001-QINU