Difference between revisions of "Part:BBa K255004:Experience"
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| − | This experience page is provided so that any user may enter their experience using this part.<BR> | + | This experience page is provided so that any user may enter their experience using this part.<BR> |
| − | + | BBa_K255004 represents a open loop system with constitutive expression of | |
| + | LacI and replication of plasmid copy. Both LacI and plasmid copy number | ||
| + | are independent of IPTG concentration (inducer). The copy number is | ||
| + | characterized by YFP while LacI is characterized by LacI-CFP fusion | ||
| + | protein. YFP was measured using FACS and the distribution obtained was | ||
| + | independent of IPTG. Beta-galactosidase was also measured at various | ||
| + | lactose concentration to characterize the expression of gene in the host | ||
| + | genome. Further the growth of the transformant was also characterized at | ||
| + | different lactose concentrations. | ||
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| + | |||
| + | ===Applications of BBa_K255004=== | ||
| + | This part was used as open loop system for our experiments. | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K255004 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K255004 StartReviews</partinfo> | ||
Revision as of 16:04, 31 October 2009
This experience page is provided so that any user may enter their experience using this part.
BBa_K255004 represents a open loop system with constitutive expression of
LacI and replication of plasmid copy. Both LacI and plasmid copy number
are independent of IPTG concentration (inducer). The copy number is
characterized by YFP while LacI is characterized by LacI-CFP fusion
protein. YFP was measured using FACS and the distribution obtained was
independent of IPTG. Beta-galactosidase was also measured at various
lactose concentration to characterize the expression of gene in the host
genome. Further the growth of the transformant was also characterized at
different lactose concentrations.
Applications of BBa_K255004
This part was used as open loop system for our experiments.
User Reviews
UNIQd79fb15dd774119c-partinfo-00000000-QINU UNIQd79fb15dd774119c-partinfo-00000001-QINU