Difference between revisions of "Part:BBa K255004"
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<partinfo>BBa_K255004 short</partinfo>
 
<partinfo>BBa_K255004 short</partinfo>
  
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network without any feedback and feedforward loop, present in pSB1A2 vector.  
+
Network without any feedback and feedforward loop, present in pSB1A2 vector. BBa_K255004 represents a open loop system with constitutive expression of
 +
LacI and replication of plasmid copy. Both LacI and plasmid copy number
 +
are independent of IPTG concentration (inducer). The copy number is
 +
characterized by YFP while LacI is characterized by LacI-CFP fusion
 +
protein.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:45, 31 October 2009

pTet.lacI-cfp(fusion).terminator.pTet.yfp.terminator

Network without any feedback and feedforward loop, present in pSB1A2 vector. BBa_K255004 represents a open loop system with constitutive expression of LacI and replication of plasmid copy. Both LacI and plasmid copy number are independent of IPTG concentration (inducer). The copy number is characterized by YFP while LacI is characterized by LacI-CFP fusion protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1161
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]