Difference between revisions of "Part:BBa K249017"
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<partinfo>BBa_K249017 short</partinfo> | <partinfo>BBa_K249017 short</partinfo> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K249017 SequenceAndFeatures</partinfo> | <partinfo>BBa_K249017 SequenceAndFeatures</partinfo> | ||
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| + | This part was designed as an inversion device for YFP fluorescence. Upon addition of Arabinose the fluorescence of the protein was expected to drop significantly. This was observed. | ||
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| + | [[Image:Results fig 11.png]] [[Image:Results fig 21.png]] | ||
Revision as of 15:05, 31 October 2009
N-terminal YFP --> Tet inverter
Arabinose repressible Tetracycline inversion of YFP with N-terminal Fusion Arganine Tag for targetting into Lumazine Microcompartment. Has a medium RBS and a double terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
This part was designed as an inversion device for YFP fluorescence. Upon addition of Arabinose the fluorescence of the protein was expected to drop significantly. This was observed.
File:Results fig 11.png File:Results fig 21.png
