Difference between revisions of "Part:BBa K5291052:Design"

 
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===References===
 
===References===
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Kuyukina, M. S., et al. (2015). "Trehalolipid biosurfactants from nonpathogenic Rhodococcus actinobacteria with diverse immunomodulatory activities." New Biotechnology 32(6): 559-568.

Latest revision as of 07:49, 2 October 2024


PEBP-PEase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 109
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2243
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2557
    Illegal NgoMIV site found at 2926
    Illegal AgeI site found at 1225
    Illegal AgeI site found at 2209
    Illegal AgeI site found at 2272
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Some restriction enzyme cutting sites are added between the sequence so that we can change the sequences of difference core of fusion protein easily. However the sequence and the expression way of this part need to be optimized


Source

The signal peptide and autotranspoter sequences come from Pseudomonas aeruginosa genomic sequence. The PEBP sequence is predicted by modeling.

References

Kuyukina, M. S., et al. (2015). "Trehalolipid biosurfactants from nonpathogenic Rhodococcus actinobacteria with diverse immunomodulatory activities." New Biotechnology 32(6): 559-568.