Difference between revisions of "Part:BBa K249014:Design"

m (→‎Design Notes)
(→‎Design Notes)
 
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Standard Prefix and Suffix, although there is BioFusion (aka Silver Lab) scar sequence fusing the YFP gene to the C-terminal Arginine Tag  
+
Standard Prefix and Suffix, although there is BioFusion (aka Silver Lab) scar sequence fusing the YFP gene to the C-terminal Arginine Tag
−
 
+
−
This part was designed as an inversion device for YFP fluorescence. Upon addition of Arabinose the fluorescence of the protein was expected to drop significantly. This was observed.
+
−
 
+
−
[[Image:Results fig 11.png]] [[Image:Results fig 21.png]]
+
  
 
===Source===
 
===Source===

Latest revision as of 15:04, 31 October 2009

C-terminal YFP --> Tet inverter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Standard Prefix and Suffix, although there is BioFusion (aka Silver Lab) scar sequence fusing the YFP gene to the C-terminal Arginine Tag

Source

BioBrick Registry and synthesized Arganine Tag

References