Difference between revisions of "Part:BBa K5443002"

 
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<partinfo>BBa_K5443002 short</partinfo>
 
<partinfo>BBa_K5443002 short</partinfo>
  
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Sam5 converts 4-coumaric acid to caffeic acid, adding the caffeic hydroxyl group necessary for subsequent modifications in the pathway.
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This enzyme from Saccharothrix espanaensis catalyzes the hydroxylation of 4-coumaric acid to make caffeic acid.  This is the second step of the vanillin biosynthesis pathway. This part builds on the work of earlier iGEM teams, who contributed C3H Parts as follows: BBa_I742105,  BBa_K238007,  BBa_K2906013.  
  
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https://static.igem.wiki/teams/5443/c3h-reaction.jpg
  
 
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Revision as of 07:31, 2 October 2024


Coumaric acid 3-hydroxylase (C3H)

This enzyme from Saccharothrix espanaensis catalyzes the hydroxylation of 4-coumaric acid to make caffeic acid. This is the second step of the vanillin biosynthesis pathway. This part builds on the work of earlier iGEM teams, who contributed C3H Parts as follows: BBa_I742105, BBa_K238007, BBa_K2906013.

c3h-reaction.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 492
  • 1000
    COMPATIBLE WITH RFC[1000]