Difference between revisions of "Part:BBa K5378006"

 
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TynA is a periplasmic amine oxidase containing copper and topaquinone cofactors, which converts amines to aldehydes.TynA-G494S discovered by Rottinghaus, A. G. et al converts phenethylamine (PEA) to phenylacetaldehyde (PAG) specifically.
 
  
 
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<partinfo>BBa_K5378006 parameters</partinfo>
 
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    <title>Usage and Biology</title>
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<h1>Usage and Biology</h1>
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<p>TynA is a periplasmic amine oxidase containing copper and topaquinone cofactors, which converts amines to aldehydes.TynA-G494S discovered by Rottinghaus, A. G. et al converts phenethylamine (PEA) to phenylacetaldehyde (PAG) specifically.</p>
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<div style="text-align:center;">
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    <img id="image" src="https://static.igem.wiki/teams/5378/safety/gsss.webp" width="50%" style="display:block; margin:auto;" alt="example" />
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<div style="text-align:center;">
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        <caption>
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            <b>Figure 1. </b> CAPTION_HERE
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<h1>Functional Verification</h1>
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<p></p>
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<div style="text-align:center;">
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    <img id="image" src="https://static.igem.wiki/teams/5378/part/2gs.webp" width="50%" style="display:block; margin:auto;" alt="example" />
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<div style="text-align:center;">
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        <caption>
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            <b>Figure 2. </b> CAPTION_HERE
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        </caption>
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<h1>Reference</h1>
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<p>[1]Rottinghaus, A. G., Xi, C., Amrofell, M. B., Yi, H., & Moon, T. S. (2022). Engineering ligand-specific biosensors for aromatic amino acids and neurochemicals. Cell systems, 13(3), 204-214.</p>
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Revision as of 07:25, 2 October 2024


tynA-G494S

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 267
    Illegal AgeI site found at 522
    Illegal AgeI site found at 2074
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Usage and Biology

TynA is a periplasmic amine oxidase containing copper and topaquinone cofactors, which converts amines to aldehydes.TynA-G494S discovered by Rottinghaus, A. G. et al converts phenethylamine (PEA) to phenylacetaldehyde (PAG) specifically.

example
Figure 1. CAPTION_HERE

Functional Verification

example
Figure 2. CAPTION_HERE

Reference

[1]Rottinghaus, A. G., Xi, C., Amrofell, M. B., Yi, H., & Moon, T. S. (2022). Engineering ligand-specific biosensors for aromatic amino acids and neurochemicals. Cell systems, 13(3), 204-214.