Difference between revisions of "Part:BBa K257025:Design"
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Latest revision as of 08:37, 30 October 2009
Fos : second part of the dimere Jun/Fos
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part needs to have silver standard fusion 23 at both N term and Cterm. Indeed, we use it as a passenger in an autotransporter that allows us to present fos at the outer side of the outer membrane of the cell. We designed a protein fusion with : signal peptide Sec-depedent to export fos from cytoplasm to periplasm + the passenger protein (fos)+ the linker and translocator domain of AIDA which will be anchored in the outer membrane.
Source
DNA synthetized in vitro.