Difference between revisions of "Part:BBa K5291050"
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| − | In degradation module we constructed pAB1-pS-PEBP-PEase to depolymerize PE microplastics.<br> | + | In degradation module we constructed pAB1-pS-PEBP-PEase aand pAB1-PEBP-GFP to depolymerize PE microplastics and verify the adsorption capacity of PEBP to PE by fluorescence microscopy.<br> |
| − | <html>< | + | If you want to know more details, please refer to <html><a href="https://parts.igem.org/Part:BBa_K5291051">BBa_K5291051</a><a href="https://parts.igem.org/Part:BBa_K5291052">BBa_K5291052</a></html>. |
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Revision as of 06:55, 2 October 2024
PE-binding peptide
Plastic-binding peptides are adsorbed to the plastic surface by hydrogen bonding and other intermolecular forces. Considering that PE-binding peptides（PEBP） has not been extensively studied and we did not find the sequence of PEBP in the paper, we decided to predict PEBP sequence by modeling method. We performed machine learning training; then performed special processing and tried to introduce neural networks. Finally, several predictors were given.
Usage and Biology
In degradation module we constructed pAB1-pS-PEBP-PEase aand pAB1-PEBP-GFP to depolymerize PE microplastics and verify the adsorption capacity of PEBP to PE by fluorescence microscopy.
If you want to know more details, please refer to BBa_K5291051BBa_K5291052.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]