Difference between revisions of "Part:BBa K5127018"

 
 
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This composite part combines the constitutive promotor J23119 and transcriptional unit iadCDE.
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==Team: BNDS-China 2024==
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===Design===
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To optimize the IAA degradation efficiency, we constructed a second plasmid that replaces the inducible T7 promoter with the constitutive promoter J23119 (Figure 1). This modification ensures continuous expression of the IAA degradation pathway, potentially enhancing its overall effectiveness.
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<p style="text-align:center;"><img src="https://static.igem.wiki/teams/5127/parts/j23119-iadcde1.png" width="400" height="auto"/>
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Figure 1. Plasmid design of J23119-iadCDE. Created by biorender.com.
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<p style="text-align:center;"><img src="https://static.igem.wiki/teams/5127/parts/j23119-iadcde2.jpg" width="400" height="auto"/>
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Figure 2. The agarose gel electrophoresis results of the PCR products of J23119-iadCDE construction. A, materials to construct J23119-iadCDE. B, Golden gate assembly result of J23119-iadCDE construction. The band at 7947bp in (B) indicated the success in plasmid construction.
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We require additional time to further characterize and evaluate the degradation function to ensure optimal performance and reliability in our system.
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===Usage and Biology===
 
  
 
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Latest revision as of 06:36, 2 October 2024


J23119-iadCDE

This composite part combines the constitutive promotor J23119 and transcriptional unit iadCDE.


Team: BNDS-China 2024

Design

To optimize the IAA degradation efficiency, we constructed a second plasmid that replaces the inducible T7 promoter with the constitutive promoter J23119 (Figure 1). This modification ensures continuous expression of the IAA degradation pathway, potentially enhancing its overall effectiveness.


Figure 1. Plasmid design of J23119-iadCDE. Created by biorender.com.



Figure 2. The agarose gel electrophoresis results of the PCR products of J23119-iadCDE construction. A, materials to construct J23119-iadCDE. B, Golden gate assembly result of J23119-iadCDE construction. The band at 7947bp in (B) indicated the success in plasmid construction.

We require additional time to further characterize and evaluate the degradation function to ensure optimal performance and reliability in our system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]