Difference between revisions of "Part:BBa K5332000:Experience"

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===Applications of BBa_K5332000===
 
===Applications of BBa_K5332000===
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Administering the engineered bacteria FMK designed by us to a mouse colitis model by gavage resulted in a recovery of colon length shortening, inhibition of immune cell recruitment, enrichment of beneficial bacteria in the gut, and an increase in gut microbiota diversity. The upregulation of pro-inflammatory factors and the downregulation of anti-inflammatory factors are also inhibited accordingly. The above results indicate that the gene element designed by our team can fully exert the expected results in the vector cell EcN and has good effects.
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Transfection of the plasmid into E. coli EcN synthetic engineering bacterium FMK and gavage to mice with intestinal inflammation, it can be found that the shortening of the length of the mouse colon is restored, the recruitment of immune cells is inhibited, the intestinal beneficial bacteria are enriched, the diversity of the intestinal flora is increased, and the up-regulation of pro-inflammatory factors and anti-inflammatory factors down-regulation are inhibited accordingly. The above results indicate that the gene elements designed by our team can fully exert the expected effect in the vector cell EcN with good results.
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Our elements can be used for spatiotemporal specificity to exert a mitigating effect on inflammation where the level of inflammation is high in the intestinal tract.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 06:26, 2 October 2024


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K5332000

Transfection of the plasmid into E. coli EcN synthetic engineering bacterium FMK and gavage to mice with intestinal inflammation, it can be found that the shortening of the length of the mouse colon is restored, the recruitment of immune cells is inhibited, the intestinal beneficial bacteria are enriched, the diversity of the intestinal flora is increased, and the up-regulation of pro-inflammatory factors and anti-inflammatory factors down-regulation are inhibited accordingly. The above results indicate that the gene elements designed by our team can fully exert the expected effect in the vector cell EcN with good results.

Our elements can be used for spatiotemporal specificity to exert a mitigating effect on inflammation where the level of inflammation is high in the intestinal tract.

User Reviews

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