Difference between revisions of "Part:BBa K5236015"

Revision as of 06:19, 2 October 2024

pET28a-pelB-IsPETase(K95S)-6xHis

This composite part codes for the mutated IsPETase K95S and expresses the enzyme in the extracellular space of chassis.

T7 promoter:used to regulate gene expression of recombinantproteins.

Lac operator: used to control gene expression by lactose.

RBS: the location to bind ribosome.

IsPETase K95S: This sequence encodes for a mutated IsPETase K95S and is derived from Escherichia coli.

pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane.

6xHis: a tag for the purification of protein which focus in the production of recombinant proteins.

T7 terminator: terminates transcription.

Fig.1 The result of pET28a-pelB-IsPETase(K95S)-6xHis DNA sequencing

Sequence and Features

Assembly Compatibility:

Revision as of 09:03, 1 October 2024 (view source) Allenyangbisz (Talk \| contribs) ← Older edit		Revision as of 06:19, 2 October 2024 (view source) Diarmuid (Talk \| contribs) Newer edit →
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	RBS: the location to bind ribosome.		RBS: the location to bind ribosome.
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	IsPETase K95S: This sequence encodes for a mutated IsPETase K95S and is derived from Escherichia coli.		IsPETase K95S: This sequence encodes for a mutated IsPETase K95S and is derived from Escherichia coli.