Difference between revisions of "Part:BBa K5115037"
| Line 6: | Line 6: | ||
__TOC__ | __TOC__ | ||
===Introduction=== | ===Introduction=== | ||
| + | This composite part consists of Tardigrade Desiccation Protein (TDP) and ''Ammopiptanthus nanus'' Antifreeze Protein (AnAFP). They were respectively tested by iGEM17_TUDelft in [https://parts.igem.org/Part:BBa_K2306003 BBa_K2306003] and [https://parts.igem.org/Part:BBa_K4765015 BBa_K4765015]. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | TDP, derived from tardigrades, is known for its ability to protect cellular structures during desiccation by forming a glass-like matrix, enabling survival in extreme dehydration. AnAFP, sourced from ''Ammopiptanthus nanus'', is an antifreeze protein that stabilizes cell membranes under cold stress by interacting with the membrane and calcium ions, preventing macromolecule aggregation. Together, these proteins enhance cellular resilience against both dehydration and freezing conditions. | ||
===Characterization=== | ===Characterization=== | ||
| + | ===Sequence and Features=== | ||
<!-- --> | <!-- --> | ||
Latest revision as of 06:09, 2 October 2024
TDP+AnAFP
Introduction
This composite part consists of Tardigrade Desiccation Protein (TDP) and Ammopiptanthus nanus Antifreeze Protein (AnAFP). They were respectively tested by iGEM17_TUDelft in BBa_K2306003 and BBa_K4765015.
Usage and Biology
TDP, derived from tardigrades, is known for its ability to protect cellular structures during desiccation by forming a glass-like matrix, enabling survival in extreme dehydration. AnAFP, sourced from Ammopiptanthus nanus, is an antifreeze protein that stabilizes cell membranes under cold stress by interacting with the membrane and calcium ions, preventing macromolecule aggregation. Together, these proteins enhance cellular resilience against both dehydration and freezing conditions.
Characterization
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 178
Illegal SapI.rc site found at 1047