Difference between revisions of "Part:BBa K5165004"

 
Line 5: Line 5:
 
A well-characterized inducible promoter is used to express GFP-linked PHB depolymerase (BBa_K5165000). This construct serves as a baseline comparison for BBa_K5165002 in terms of PHB depolymerase production. Baseline PHB depolymerase levels can determine the degree of which the larger construct is compromised due to the implementation of the quorum sensing system and other additional elements to achieve the two-step mechanism.  
 
A well-characterized inducible promoter is used to express GFP-linked PHB depolymerase (BBa_K5165000). This construct serves as a baseline comparison for BBa_K5165002 in terms of PHB depolymerase production. Baseline PHB depolymerase levels can determine the degree of which the larger construct is compromised due to the implementation of the quorum sensing system and other additional elements to achieve the two-step mechanism.  
  
 +
<html><img src="https://static.igem.wiki/teams/5165/average-fluorescence-talfu-gfp.png", width=600, height=500></html>
  
  

Revision as of 05:44, 2 October 2024


GFP-linked PHB Depolymerase (IPTG inducible promoter)

A well-characterized inducible promoter is used to express GFP-linked PHB depolymerase (BBa_K5165000). This construct serves as a baseline comparison for BBa_K5165002 in terms of PHB depolymerase production. Baseline PHB depolymerase levels can determine the degree of which the larger construct is compromised due to the implementation of the quorum sensing system and other additional elements to achieve the two-step mechanism.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 697
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 634
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 662