Difference between revisions of "Part:BBa K257010:Design"

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===Design Notes===
 
===Design Notes===
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To avoid frameshift between clyA fusion C Term and RFP fusion N Term we use respectively the fusion silver standard suffix RFC 23 (for clyA C term, the prefix being standard RFC 10) and the fusion silver standard prefix RFC 23 (for RFP N Term, the suffix being standard RFC 10)
+
To avoid frameshift between clyA fusion C Term and RFP fusion N Term we use respectively the fusion silver standard suffix 23 (for clyA C term, the prefix being standard 10) and the fusion silver standard prefix 23 (for RFP N Term, the suffix being standard 10)
  
 
===Source===
 
===Source===

Latest revision as of 21:04, 25 October 2009

clyA + RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 84
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1494
    Illegal AgeI site found at 1606
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To avoid frameshift between clyA fusion C Term and RFP fusion N Term we use respectively the fusion silver standard suffix 23 (for clyA C term, the prefix being standard 10) and the fusion silver standard prefix 23 (for RFP N Term, the suffix being standard 10)

Source

Assembly of BBa_K257002 and BBa_K257008.

References