Difference between revisions of "Part:BBa K5201001"
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<p><b>Biology</b> </p> | <p><b>Biology</b> </p> | ||
<p><i>kfiD </i>encodes UDP-glucose-6-dehydrogenase (UGDH) from <i>E. coli K5</i> strain. </p> | <p><i>kfiD </i>encodes UDP-glucose-6-dehydrogenase (UGDH) from <i>E. coli K5</i> strain. </p> | ||
Revision as of 05:32, 2 October 2024
kfiD codons are optimized for overexpression of UDP-glucose-6-dehyrogenase
Characterisation of BBa_K5201001: HongKong-UCCKE
Biology
kfiD encodes UDP-glucose-6-dehydrogenase (UGDH) from E. coli K5 strain.
Usage and design
UGDH is endogenously expressed in a low level in E. coli, our recombinant plasmid will overexpress UGDH under an inducible promoter (BBa_R0010). We hope that overexpression of kfiD can increase production of Hyaluronic Acid, by converting UDP-glucose to UDP-glucuronic acid, one of the precursors of HA. HA is widely utilized by the cosmetic industry for its significant water absorption and retention properties. And therefore, we want to further implement it for agricultural use.