Difference between revisions of "Part:BBa K239005"

Revision as of 16:51, 24 October 2009

NarK promoter, Fnr activated under anaerobic conditions

Sequence contains 2 Fnr, 1 Fis, 4 NarL and 1 IHF binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions.

Area of application: Detection of when the cell is switching to anaerobic metabolism.

Experiments by Kolesnikow et al. (1992) have showed that NarK expression is upregulated 100-fold under anaerobic condtitions and a further 8-fold in the presence of nitrate.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 15:41, 20 October 2009 (view source) Axelnystrom (Talk \| contribs) ← Older edit		Revision as of 16:51, 24 October 2009 (view source) Axelnystrom (Talk \| contribs) Newer edit →
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	Area of application: Detection of when the cell is switching to anaerobic metabolism.		Area of application: Detection of when the cell is switching to anaerobic metabolism.

−	Experiments by Kolesnikow et al. (1992) ~~has~~ showed that NarK expression is upregulated 100-fold under anaerobic condtitions and a further 8-fold in the presence of nitrate.	+	Experiments by Kolesnikow et al. (1992) have showed that NarK expression is upregulated 100-fold under anaerobic condtitions and a further 8-fold in the presence of nitrate.