Difference between revisions of "Part:BBa K5180777"
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===Images and Figures===
 
===Images and Figures===
  
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'''Figure 1: Analysis of Dependent Calprotectin Production'''
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[[File:calprotectindependentlysis.jpg|thumb|400px|This image shows the analysis of dependent calprotectin production using the quorum sensing system.]]
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        <img src="https://static.igem.wiki/teams/5180/registry/calprotectindependentlysis.jpg" style="width:400px;">
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        <center><b>Figure 1: </b>Analysis of Dependent Calprotectin Production</center>
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        Figure 1 demonstrates the impact of calprotectin-dependent lysis. Increases in calprotectin concentration result in zinc deficiency, which can then be detected by the ykgMO promoter. When there is a high concentration of zinc, this pathway is suppressed, showing the feedback mechanism of calprotectin in controlling zinc homeostasis.
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===Usage and Biology===
 
===Usage and Biology===
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<partinfo>BBa_K5180777 parameters</partinfo>
 
<partinfo>BBa_K5180777 parameters</partinfo>
 
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Revision as of 04:08, 2 October 2024


LuxI_LuxR_Plux_rzrz1

This construct integrates the luxI/luxR quorum sensing system to drive the inducible production of the rzrz01 lysis gene in Escherichia coli. Modeled after the quorum sensing system of Vibrio fischeri, this design enables cell-to-cell communication, ultimately triggering cell lysis. When the luxI gene synthesizes the signaling molecule acyl homoserine lactone (AHL), it binds to the LuxR regulator. The LuxR-AHL complex then interacts with the Plux promoter, activating the transcription of the rzrz01 lysis gene. This results in controlled self-lysis of the bacterial cells in response to AHL accumulation.

Images and Figures

Figure 1: Analysis of Dependent Calprotectin Production
Figure 1 demonstrates the impact of calprotectin-dependent lysis. Increases in calprotectin concentration result in zinc deficiency, which can then be detected by the ykgMO promoter. When there is a high concentration of zinc, this pathway is suppressed, showing the feedback mechanism of calprotectin in controlling zinc homeostasis.
<figure> Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 829
    Illegal NheI site found at 852
    Illegal NheI site found at 2933
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1076
    Illegal BamHI site found at 3042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]