Difference between revisions of "Part:BBa K5291048"

Revision as of 03:49, 2 October 2024

AlkB2

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Usage and Biology

AlkB2 is from the system AlkB2-Rd45-AdhA, which is designed to accelerate the degradation rate by improving the absorbing and hydroxylation of PE monomer.

Fig.1 The map of the plasmid pAB1-AlkB2-Rd45-AdhA.

Fig.2 The PCR result of AlkB-Rd45-AdhA fragment. The band was identycal to the expected length of 3033bp.

And we have conducted SDS-page of pAB1-AlkB2-Rd45-AdhA, which is expected to express a large fusion protein AlkB2-Rd45-AdhA with a molecular weight of 94.59kDa. In the figure, it can be seen that there were an extra bands in the experimental group than in the control group at a position slightly less than 100kDa, suggesting that AlkB2-Rd45-AdhA protein was successfully expressed.

Fig.3 SDS-PAGE result of AlkB2-Rd45-AdhA.

Since we set AlkB2, Rd45 and AdhA as a whole, the overall functions need moving to the part BBa_K5291033.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 657
1000
COMPATIBLE WITH RFC[1000]

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 AlkB2 is from the system AlkB2-Rd45-AdhA, which is designed to accelerate the degradation rate by improving the absorbing and hydroxylation of PE monomer.<br><br>
-<html><img width = "550" src="https://static.igem.wiki/teams/5291/images/part-wyn/map-alkb-adh.png" /></html>(lack)
+<html><img width = "550" src="https://static.igem.wiki/teams/5291/images/part-wyn/map-alkb-adh.png" /></html>
 <br><b>Fig.1 The map of the plasmid pAB1-AlkB2-Rd45-AdhA.</b><br><br>
 <html><img width = "600" src="https://static.igem.wiki/teams/5291/images/part-wyn/alkb-adh-schema.png" /></html>