Difference between revisions of "Part:BBa M36920"
 
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Promoter sequence with recognition site for ZntR transcriptional regulator protein. ZntR activates transcription when Zn(II) is bound. In the absence of Zinc, the ZntR protein will bind to this promoter region, causing a halt in transcription. In the presence of zinc, the ZntR protein will undergo conformation and unbind from the promoter region, allowing transcription. This part was edited from Part:BBa_K190016 by sequence changes from DNA2.0 for optimization.
 
Promoter sequence with recognition site for ZntR transcriptional regulator protein. ZntR activates transcription when Zn(II) is bound. In the absence of Zinc, the ZntR protein will bind to this promoter region, causing a halt in transcription. In the presence of zinc, the ZntR protein will undergo conformation and unbind from the promoter region, allowing transcription. This part was edited from Part:BBa_K190016 by sequence changes from DNA2.0 for optimization.
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=== Contribution from Squirrel-Beijing II  2024 ===
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ZntR and Zntp (BBa_K190016) are endogenous zinc ion sensing systems of E. coli. We utilized a dual plasmid system to test the expression levels of different promoters for ZntR. The promoter library was sourced from endogenous E. coli promoters and purchased from Ailurus.
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<img src="https://static.igem.wiki/teams/5456/part-registry/trancription-unit-2.png" alt="图1" style="width: 350px; margin-right: 10px;">
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<img src="https://static.igem.wiki/teams/5456/part-registry/trancription-unit-3.png" alt="图1" style="width: 350px; margin-right: 10px;">
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We induced the system with 0 µM and 500 µM concentrations, respectively, and obtained their growth curves.：
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  <div style="display: flex; justify-content: center; align-items: center;">
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<img src="https://static.igem.wiki/teams/5456/part-registry/combined-growth-curves-zn-0-02-96split-no-legend.png" alt="图1" style="width: 350px; margin-right: 10px;">
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<img src="https://static.igem.wiki/teams/5456/part-registry/combined-growth-curves-zn-500-02-96split-no-legend.png" alt="图1" style="width: 350px; margin-right: 10px;">
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</div>
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By dividing the signal values after the two systems reached a steady state, we obtained the ratio of system fluorescence values, which we used to measure the strength of the different promoters in initiating the expression of zntR.
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<html>
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  <div style="display: flex; justify-content: center; align-items: center;">
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<img src="https://static.igem.wiki/teams/5456/part-registry/zhutu.png" alt="图1" style="width: 350px; margin-right: 10px;">
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<img src="https://static.igem.wiki/teams/5456/part-registry/ratio.png" alt="图1" style="width: 350px; margin-right: 10px;">
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We hope this will provide a reference for other iGEM teams in the future.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:38, 2 October 2024

Zinc Promoter (ZntR regulated)

Promoter sequence with recognition site for ZntR transcriptional regulator protein. ZntR activates transcription when Zn(II) is bound. In the absence of Zinc, the ZntR protein will bind to this promoter region, causing a halt in transcription. In the presence of zinc, the ZntR protein will undergo conformation and unbind from the promoter region, allowing transcription. This part was edited from Part:BBa_K190016 by sequence changes from DNA2.0 for optimization.

Contribution from Squirrel-Beijing II 2024

ZntR and Zntp (BBa_K190016) are endogenous zinc ion sensing systems of E. coli. We utilized a dual plasmid system to test the expression levels of different promoters for ZntR. The promoter library was sourced from endogenous E. coli promoters and purchased from Ailurus.

图1 图1
We induced the system with 0 µM and 500 µM concentrations, respectively, and obtained their growth curves.：
图1 图1
By dividing the signal values after the two systems reached a steady state, we obtained the ratio of system fluorescence values, which we used to measure the strength of the different promoters in initiating the expression of zntR.

图1 图1
We hope this will provide a reference for other iGEM teams in the future.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]