Difference between revisions of "Part:BBa K5233010:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | PtphC need tphR to regulated, | + | PtphC need tphR to be regulated, we connect it with a reporter gene downstream to validate the presence of TPA and the degradation effect. |
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===Source=== | ===Source=== | ||
Latest revision as of 03:12, 2 October 2024
PtphC, a kind of promter that responds to the presence of TPA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 80
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 80
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 80
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 80
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PtphC need tphR to be regulated, we connect it with a reporter gene downstream to validate the presence of TPA and the degradation effect.
Source
It comes from Comamonas thiooxidans strain S23.
References
Dierkes RF, Wypych A, Pérez-García P, Danso D, Chow J, Streit WR. An Ultra-Sensitive Comamonas thiooxidans Biosensor for the Rapid Detection of Enzymatic Polyethylene Terephthalate (PET) Degradation. Appl Environ Microbiol. 2023 Jan 31;89(1):e0160322. doi: 10.1128/aem.01603-22. Epub 2022 Dec 12. PMID: 36507653; PMCID: PMC9888244.