Difference between revisions of "Part:BBa K5291049"
(→‎Usage and Biology)
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===Usage and Biology===
 
===Usage and Biology===
  
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<i>AdhA</i> is from the system AlkB2-Rd45-AdhA, which is designed to accelerate the degradation rate by improving the absorbing and hydroxylation of PE monomer.<br><br>
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AdhA is from the system AlkB2-Rd45-AdhA, which is designed to accelerate the degradation rate by improving the absorbing and hydroxylation of PE monomer.<br><br>
 
<html><img width = "700" src="" /></html>(lack)
 
<html><img width = "700" src="" /></html>(lack)
 
<br><b>Fig.1 The map of the plasmid pAB1-AlkB2-Rd45-AdhA.</b><br><br>
 
<br><b>Fig.1 The map of the plasmid pAB1-AlkB2-Rd45-AdhA.</b><br><br>
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<html><img width = "600" src="https://static.igem.wiki/teams/5291/images/part-wyn/sdspage-alkb-adh.png" /></html>
 
<html><img width = "600" src="https://static.igem.wiki/teams/5291/images/part-wyn/sdspage-alkb-adh.png" /></html>
 
<br><b>Fig.3 SDS-PAGE result of AlkB2-Rd45-AdhA.</b><br><br>
 
<br><b>Fig.3 SDS-PAGE result of AlkB2-Rd45-AdhA.</b><br><br>
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Since we set AlkB<sub>2</sub> Rd45 and AdhA as a whole, the overall functions need moving to the part BBa_K5291033.
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Since we set AlkB2 Rd45 and AdhA as a whole, the overall functions need moving to the part BBa_K5291033.
  
 
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Revision as of 03:12, 2 October 2024


AdhA

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Usage and Biology

AdhA is from the system AlkB2-Rd45-AdhA, which is designed to accelerate the degradation rate by improving the absorbing and hydroxylation of PE monomer.

(lack)
Fig.1 The map of the plasmid pAB1-AlkB2-Rd45-AdhA.


Fig.2 The PCR result of AlkB-Rd45-AdhA fragment. The band was identycal to the expected length of 3033bp.

And we have conducted SDS-page of pAB1-AlkB2-Rd45-AdhA, which is expected to express a large fusion protein AlkB2-Rd45-AdhA with a molecular weight of 94.59kDa. In the figure, it can be seen that there were an extra bands in the experimental group than in the control group at a position slightly less than 100kDa, suggesting that AlkB2-Rd45-AdhA protein was successfully expressed.

Fig.3 SDS-PAGE result of AlkB2-Rd45-AdhA.

Since we set AlkB2 Rd45 and AdhA as a whole, the overall functions need moving to the part BBa_K5291033.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 769
  • 1000
    COMPATIBLE WITH RFC[1000]