Difference between revisions of "Part:BBa K5325002:Design"

(→‎Design Notes)
(→‎Source)
 
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===Source===
 
===Source===
  
−
The gene is taken from the genome of <i>Rhodopseudomonas palustris</i>.
+
The part originates from the DNA sequence of <i>Rhodopseudomonas palustris</i> CGA009 complete sequence.
 +
<br>NCBI ID and link to NCBI profile: <HTML><a href="https://www.ncbi.nlm.nih.gov/nuccore/NC_005296.1?report=genbank&from=1678194&to=1679081">NC_005296.1</a></html>
  
 
===References===
 
===References===
 
Hajighasemi, M., Nocek, B.P., <i>et al.</i> (2016). "Biochemical and Structural Insights into Enzymatic Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases." Biomacromolecules 17(6):2027-2039
 
Hajighasemi, M., Nocek, B.P., <i>et al.</i> (2016). "Biochemical and Structural Insights into Enzymatic Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases." Biomacromolecules 17(6):2027-2039

Latest revision as of 03:03, 2 October 2024


RPA1511_plaA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 70
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 820


Design Notes

Addition of the FLAG tag that aids protein purification and visualization on Western Blot
DNA sequence was codon optimized for expression in E. coli and Shewanella oneidensis.

Source

The part originates from the DNA sequence of Rhodopseudomonas palustris CGA009 complete sequence.
NCBI ID and link to NCBI profile: NC_005296.1

References

Hajighasemi, M., Nocek, B.P., et al. (2016). "Biochemical and Structural Insights into Enzymatic Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases." Biomacromolecules 17(6):2027-2039