Difference between revisions of "Part:BBa K208006:Design"

(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
HlyA secretion tag.  Silver fusion compatible. In pSB3K3.
+
This part was designed for Silver-fusion compatibility, and can accordingly be fused to any Silver-fusion protein. It is in plasmid pSB3K3.  
  
 
===Source===
 
===Source===
  
−
Synthetic
+
This part was designed to have the Silver-fusion prefix and suffix, and was synthetically produced by DNA 2.0. The obtained plasmid was then cut with EcoRI and SpeI. The insert was then ligated with a BioBrick compatible vector, pSB3K3. This part has been sequenced.
  
 
===References===
 
===References===
 +
1. Binet,R, Letoffe S, Ghigo JM, Delepelaire P, Wandersman C. Protein secretion by Gram-negative bacterial ABC exporters-a review. Gene 1997;192:7-11<br>
 +
2. Blight MA, Holland IB (1994) Heterologous protein secretion and the versatile Escherichia coli haemolysin translocator. Trends Biotechnol 12:450-455<br>
 +
3. Desveaux M, Parham N, Scott-Tucker A, Henderson IR (2004) The general secretory pathway: a general misnomer. Trends Micro 12:306-309<Br>
 +
4. Gentschev I, Goebel W (2003) Type I protein secretion systems in gram-negative bacteria: Escherichia coli alpha-hemolysin secretion. In: Oudega B, editor. Kluwer Academic Publishers, pp.121-139<br>
 +
5. Hess J, Gentschev I, Goebel W, Jarchau T (1990) Analysis of the haemolysin secretion system by PhoA-HlyA fusion proteins. Mol Gen Genet 224:201-208<br>
 +
6. Mergulhão, FJM, Summers DK, Monteiro (2005) Recombinant protein secretion in Escherichia coli Biotechnol Adv 23:177-202<br>
 +
7. Sapriel G, Wandersman C, Delepelaire P (2003) The SecB chaperone is bifnctional in Serratia marcescens: SecB is involved in the Sec pathway and required for HasA secretion by the ABC transporter. J Bacteriol 185:80-88<br>

Latest revision as of 00:57, 23 October 2009

HlyA Signal Peptide - Silver Fusion Compatible


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed for Silver-fusion compatibility, and can accordingly be fused to any Silver-fusion protein. It is in plasmid pSB3K3.

Source

This part was designed to have the Silver-fusion prefix and suffix, and was synthetically produced by DNA 2.0. The obtained plasmid was then cut with EcoRI and SpeI. The insert was then ligated with a BioBrick compatible vector, pSB3K3. This part has been sequenced.

References

1. Binet,R, Letoffe S, Ghigo JM, Delepelaire P, Wandersman C. Protein secretion by Gram-negative bacterial ABC exporters-a review. Gene 1997;192:7-11
2. Blight MA, Holland IB (1994) Heterologous protein secretion and the versatile Escherichia coli haemolysin translocator. Trends Biotechnol 12:450-455
3. Desveaux M, Parham N, Scott-Tucker A, Henderson IR (2004) The general secretory pathway: a general misnomer. Trends Micro 12:306-309
4. Gentschev I, Goebel W (2003) Type I protein secretion systems in gram-negative bacteria: Escherichia coli alpha-hemolysin secretion. In: Oudega B, editor. Kluwer Academic Publishers, pp.121-139
5. Hess J, Gentschev I, Goebel W, Jarchau T (1990) Analysis of the haemolysin secretion system by PhoA-HlyA fusion proteins. Mol Gen Genet 224:201-208
6. Mergulhão, FJM, Summers DK, Monteiro (2005) Recombinant protein secretion in Escherichia coli Biotechnol Adv 23:177-202
7. Sapriel G, Wandersman C, Delepelaire P (2003) The SecB chaperone is bifnctional in Serratia marcescens: SecB is involved in the Sec pathway and required for HasA secretion by the ABC transporter. J Bacteriol 185:80-88