Difference between revisions of "Part:BBa K5291048:Design"

 
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===Design Notes===
 
===Design Notes===
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In degradation module we constructed 4 main plasmids. Two PE degrading enzymes, CYPY96F-VHB and AlkB2-Rd45-AdhA, are designed to accelerate the degradation rate by improving the absorbing and hydroxylation of PE monomer. In order to express above two enzymes in our chassis， pAB1-CYPY96F-VHB and pAB1-AlkB2-Rd45-VHB were constructed. pAB1-pS-PEBP-PEase was constructed to depolymerize PE microplastics. In addition, pAB1-PEBP-GFP was constructed to verify the adsorption capacity of PEBP to PE by fluorescence microscopy. AlkB<sub>2</sub> is a significant component in degradation module.
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===Source===
 
===Source===

Revision as of 02:49, 2 October 2024


AlkB2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 657
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In degradation module we constructed 4 main plasmids. Two PE degrading enzymes, CYPY96F-VHB and AlkB2-Rd45-AdhA, are designed to accelerate the degradation rate by improving the absorbing and hydroxylation of PE monomer. In order to express above two enzymes in our chassis， pAB1-CYPY96F-VHB and pAB1-AlkB2-Rd45-VHB were constructed. pAB1-pS-PEBP-PEase was constructed to depolymerize PE microplastics. In addition, pAB1-PEBP-GFP was constructed to verify the adsorption capacity of PEBP to PE by fluorescence microscopy. AlkB2 is a significant component in degradation module.

Source

Pseudomonas aeruginosa PAO1

References