Difference between revisions of "Part:BBa K5233008:Design"
Lambertbai (Talk | contribs) |
(→References) |
||
| Line 17: | Line 17: | ||
===References=== | ===References=== | ||
| + | Dierkes RF, Wypych A, Pérez-García P, Danso D, Chow J, Streit WR. An Ultra-Sensitive Comamonas thiooxidans Biosensor for the Rapid Detection of Enzymatic Polyethylene Terephthalate (PET) Degradation. Appl Environ Microbiol. 2023 Jan 31;89(1):e0160322. doi: 10.1128/aem.01603-22. Epub 2022 Dec 12. PMID: 36507653; PMCID: PMC9888244. | ||
Latest revision as of 02:47, 2 October 2024
tphC, a kind of substrate-binding protein (SBP) belonging to the tripartite tricarboxylate transport
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 808
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 808
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 237
Illegal BamHI site found at 976 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 808
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 808
Illegal NgoMIV site found at 735
Illegal AgeI site found at 70
Illegal AgeI site found at 529 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Wild E. coli has low ability to transfer TPA into its body, so we transformed E. coli with plasmids carrying tpiA, tpiB, and tphC to transport TPA.
Source
It comes from Comamonas thiooxidans strain S23.
References
Dierkes RF, Wypych A, Pérez-García P, Danso D, Chow J, Streit WR. An Ultra-Sensitive Comamonas thiooxidans Biosensor for the Rapid Detection of Enzymatic Polyethylene Terephthalate (PET) Degradation. Appl Environ Microbiol. 2023 Jan 31;89(1):e0160322. doi: 10.1128/aem.01603-22. Epub 2022 Dec 12. PMID: 36507653; PMCID: PMC9888244.