Difference between revisions of "Part:BBa K5233007:Design"
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===References=== | ===References=== | ||
| + | Dierkes RF, Wypych A, Pérez-García P, Danso D, Chow J, Streit WR. An Ultra-Sensitive Comamonas thiooxidans Biosensor for the Rapid Detection of Enzymatic Polyethylene Terephthalate (PET) Degradation. Appl Environ Microbiol. 2023 Jan 31;89(1):e0160322. doi: 10.1128/aem.01603-22. Epub 2022 Dec 12. PMID: 36507653; PMCID: PMC9888244. | ||
Latest revision as of 02:45, 2 October 2024
tpiB, a kind of tripartite tricarboxylate transporter(TTT) family protein
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 457
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 457
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 496
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 457
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 457
Illegal AgeI site found at 103 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Wild E. coli has low ability to transfer TPA into its body, so we transformed E. coli with plasmids carrying tpiA, tpiB, and tphC to transport TPA.
Source
It comes from Comamonas thiooxidans strain S23.
References
Dierkes RF, Wypych A, Pérez-García P, Danso D, Chow J, Streit WR. An Ultra-Sensitive Comamonas thiooxidans Biosensor for the Rapid Detection of Enzymatic Polyethylene Terephthalate (PET) Degradation. Appl Environ Microbiol. 2023 Jan 31;89(1):e0160322. doi: 10.1128/aem.01603-22. Epub 2022 Dec 12. PMID: 36507653; PMCID: PMC9888244.