Difference between revisions of "Part:BBa K5302028"

 
 
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This work is derived from pBBR1MCS-INP-mCherry, pUC19-miniZ-Z3C, and it has undergone codon optimization. This composite part combines INP(about 30kda) and Z3C(7kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express Z3C. The plasmid uses lac promotor and has kanamycin resistence.
 
This work is derived from pBBR1MCS-INP-mCherry, pUC19-miniZ-Z3C, and it has undergone codon optimization. This composite part combines INP(about 30kda) and Z3C(7kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express Z3C. The plasmid uses lac promotor and has kanamycin resistence.
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Z3C is derived from three helix 58-residue Z-domain of staphylococcal protein A, but it has three helix. Z3C also shows great affinity with VEGF(KD=41 nM). We used pBBR plasmid as a backbone and transfered Z3C into Escherichia coli Nissle 1917, and finally succeeded in expressing Z3C.
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-z3c-1.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 1. </b> sequence of Z3C and its KD with VEGF
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        </caption>
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    </div>
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<div style="text-align:center;">
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-z3c-2.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 2. </b> Cartoon representation of the crystal structure of the mini-Z highlighting randomized residues shown as sticks and coloring the helices
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        </caption>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:03, 2 October 2024


pBBR-INP-Z3C

This work is derived from pBBR1MCS-INP-mCherry, pUC19-miniZ-Z3C, and it has undergone codon optimization. This composite part combines INP(about 30kda) and Z3C(7kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express Z3C. The plasmid uses lac promotor and has kanamycin resistence.

Z3C is derived from three helix 58-residue Z-domain of staphylococcal protein A, but it has three helix. Z3C also shows great affinity with VEGF(KD=41 nM). We used pBBR plasmid as a backbone and transfered Z3C into Escherichia coli Nissle 1917, and finally succeeded in expressing Z3C.

Jamboree Program
Figure 1. sequence of Z3C and its KD with VEGF

Jamboree Program
Figure 2. Cartoon representation of the crystal structure of the mini-Z highlighting randomized residues shown as sticks and coloring the helices

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4458
    Illegal XbaI site found at 3189
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4458
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal NotI site found at 1057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4458
    Illegal BglII site found at 1803
    Illegal BamHI site found at 3195
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4458
    Illegal XbaI site found at 3189
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4458
    Illegal XbaI site found at 3189
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal NgoMIV site found at 2467
    Illegal NgoMIV site found at 2750
    Illegal NgoMIV site found at 3616
    Illegal NgoMIV site found at 5123
    Illegal AgeI site found at 4963
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1379
    Illegal SapI.rc site found at 2316
    Illegal SapI.rc site found at 2526