Difference between revisions of "Part:BBa K5226033:Design"

 
(→‎Design Notes)
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
none
+
Although this part complies with one of the RFC 10 or Type IIS standards, it is inconsistent with the standards upheld by other basic parts. As a result, the composite component formed by their combination has illegal sites, necessitating a synonymous mutation. Mutate the base pairs at 30bp of ogdA from a/t to g/c.Mutate the base pairs at 30bp of ogdA from a/t to c/g.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Latest revision as of 01:44, 2 October 2024


ogdA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Although this part complies with one of the RFC 10 or Type IIS standards, it is inconsistent with the standards upheld by other basic parts. As a result, the composite component formed by their combination has illegal sites, necessitating a synonymous mutation. Mutate the base pairs at 30bp of ogdA from a/t to g/c.Mutate the base pairs at 30bp of ogdA from a/t to c/g.

Source

PCR amplification.Sequence source is from Synechococcus sp. PCC 7002, found through NCBI(https://www.ncbi.nlm.nih.gov/nucleotide/CP000951.1?report=genbank&log$=nuclalign&blast_rank=1&RID=CZS2C9VF013&from=2891967&to=2893603)

References