Difference between revisions of "Part:BBa K208000"
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<partinfo>BBa_K208000 short</partinfo>
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<partinfo>BBa_K208000 short</partinfo><br>
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This is a new Silver-fusion compatible GFP BioBrick part. This particular GFP was previously mutated for improved fluorescence photostability (Crameri, 1996). The excitation and emission wavelengths for this GFP are 395 nm and 509 nm, respectively. That being said, GFP-positive cells emit a bright green fluorescence when exposed to shorter-wavelength UV light, such as on a transilluminator. See part BBa_K208045 for a functional composite part containing this GFP with a Lac promoter.
  
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GFP with excitation at 395nm and emission at 509nm.  This GFP allows for easy monitoring of expression using a UV light box.
 
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===Usage and Biology===
 
===Usage and Biology===
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<br>The Silver-fusion compatibility means that the prefix and suffix are slightly modified from the original BioBrick prefix and suffix. Specifically, a single base pair is removed from the prefix and suffix, directly next to the part. <br>
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The Silver prefix is: 5' GAATTC GCGGCCGC T TCTAGA 3'  <br>
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The Silver suffix is: 5' ACTAGT A GCGGCCG CTGCAG 3' <br>
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<br>The scar that is formed from ligating XbaI and SpeI between two fusion compatible parts is 5' ACTAGA 3', which is six base pairs long and does not contain a stop codon. Therefore, fusion of two proteins can occur by this method. Additionally, this part is still useful and functional for non-fusion applications. There are more design considerations for when using Silver BioBrick parts. See this description by the Silver lab for more information about <ahref="http://www.openwetware.org/wiki/Silver:_BB_Strategy"><b><font color=#009900>Silver fusion</font></b></a>
  
 
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Revision as of 14:58, 22 October 2009

GFP
This is a new Silver-fusion compatible GFP BioBrick part. This particular GFP was previously mutated for improved fluorescence photostability (Crameri, 1996). The excitation and emission wavelengths for this GFP are 395 nm and 509 nm, respectively. That being said, GFP-positive cells emit a bright green fluorescence when exposed to shorter-wavelength UV light, such as on a transilluminator. See part BBa_K208045 for a functional composite part containing this GFP with a Lac promoter.

Usage and Biology


The Silver-fusion compatibility means that the prefix and suffix are slightly modified from the original BioBrick prefix and suffix. Specifically, a single base pair is removed from the prefix and suffix, directly next to the part.

The Silver prefix is: 5' GAATTC GCGGCCGC T TCTAGA 3'
The Silver suffix is: 5' ACTAGT A GCGGCCG CTGCAG 3'


The scar that is formed from ligating XbaI and SpeI between two fusion compatible parts is 5' ACTAGA 3', which is six base pairs long and does not contain a stop codon. Therefore, fusion of two proteins can occur by this method. Additionally, this part is still useful and functional for non-fusion applications. There are more design considerations for when using Silver BioBrick parts. See this description by the Silver lab for more information about <ahref="http://www.openwetware.org/wiki/Silver:_BB_Strategy">Silver fusion</a>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 523
    Illegal XhoI site found at 424
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]