Difference between revisions of "Part:BBa K197016:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K197016 short</partinfo>
 
<partinfo>BBa_K197016 short</partinfo>
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===Design Notes===
 
===Design Notes===
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N/A
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This part is in BglBricks Standard.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BglBricks Standard is available at:
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[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
  
 
===Source===
 
===Source===

Latest revision as of 05:19, 22 October 2009

{CPG_L2 (circularly permuted OmpG)}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 258
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

PCR ca998/O09ig278 on M10086		  		(873, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5           	(EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09cpgl2   		{<CPG_L2>}
----
ca998	Forward Oligo 
CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC
O09ig278	Reverse Oligo for the removal of stop codon from {<CPG_L2>}
CGTTAGGATCCcgcgctataatctaccggcccttcc

References