Difference between revisions of "Part:BBa K197012:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K197012 short</partinfo>
 
<partinfo>BBa_K197012 short</partinfo>
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===Design Notes===
 
===Design Notes===
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N/A
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This part is in BglBricks Standard.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BglBricks Standard is available at:
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[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
  
 
===Source===
 
===Source===

Latest revision as of 05:18, 22 October 2009

{VirG(IcsA)}


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 330
    Illegal SpeI site found at 777
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 330
    Illegal SpeI site found at 777
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 330
    Illegal SpeI site found at 777
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 330
    Illegal SpeI site found at 777
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

PCR O09ig302/O09ig301 on S. Flexneri (490bp, gp = frag1)
PCR O09ig300/O09ig303 on S. Flexneri       (986bp, gp = frag2)
----
PCR O09ig302/O09ig303 on mixed frags    (1457bp, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig302         {?VirG AtD?}
----
O09ig302    Forward PCR of {?VirG AtD?} (B09ig302)
AAGTGGAATTCATGAGATCTTCGTCAAATGTCGGTCAGAC
O09ig301    Reverse internal oligo for {?VirG AtD?} (B09ig302)
CTGCCAGCCTCAGGGCGCC
O09ig300    Forward internal oligo for {?VirG AtD?} (B09ig302)
GGCGCCCTGAGGCTGGCAG
O09ig303    Reverse PCR of {?VirG AtD?} (B09ig302)
GTTAGGGATCCGAAGGTATATTTCACACCC


References