Difference between revisions of "Part:BBa K197006:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K197006 short</partinfo>
 
<partinfo>BBa_K197006 short</partinfo>
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===Design Notes===
 
===Design Notes===
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N/A
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This part is in BglBricks Standard.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BglBricks Standard is available at:
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[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
  
 
===Source===
 
===Source===

Latest revision as of 05:16, 22 October 2009

{Pcryo_1225 AtD}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

Pool O09ig129 through O09ig136, assemble by PCA
PCR O09ig129/O09ig136 on PCA reaction        (272, EcoRI/BamHI
Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig129   {?Pcryo_1225 AtD?}
----
O09ig129     PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
CCATAGAATTCATGAGATCTGTTGAAGACGACGCTAACGCTGGT
O09ig130     PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
GCGGCAGAGAAGACATAGCCATAGCAGCAGAGATACCAGCGTTAGCGTCGTCTT
O09ig131     PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
GGCTATGTCTTCTCTGCCGCAGGCTTACATCATCGGTAAACCGATGATCGGTGG
O09ig132     PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
AACAGCAGATTCACCGTTGTAGGTAGCGATACCACCACCGATCATCGGTTTACC
O09ig133     PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
ACAACGGTGAATCTGCTGTTGCTATCGGTTTCTCTAAAATGTCTAACGACGGTC
O09ig134     PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
GTGTCAGCAGTGCCGTTCAGTTTCATAACCCAACGACCGTCGTTAGACATTTTA
O09ig135     PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
CTGAACGGCACTGCTGACACTCAAGGCAATGTAGGTGCTGCTATCGGTGCTGGT
O09ig136     PCA assembly of {?Pcryo_1225 AtD?} (B09ig129)
CGTTAGGATCCGTCGAAGTGGAAACCAGCACCGATAGCAGCAC

References