Difference between revisions of "Part:BBa K197002:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K197002 short</partinfo>
 
<partinfo>BBa_K197002 short</partinfo>
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===Design Notes===
 
===Design Notes===
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N/A
+
This part is in BglBricks Standard.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BglBricks Standard is available at:
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[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
  
 
===Source===
 
===Source===

Latest revision as of 05:14, 22 October 2009

{OprF AtD}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

Pool O09ig57 through O09ig78, assemble by PCA
PCR O09ig57/O09ig78 on PCA reaction        (710, EcoRI/BamHI
Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig57   {?OprF AtD?}
----
O09ig57     PCA assembly of {?OprF AtD?} (B09ig57)
CCATAGAATTCATGAGATCTGACTCTAAAATGACTGGCGCTTACGGTC
O09ig58     PCA assembly of {?OprF AtD?} (B09ig57)
AGAGGTAGAGTCAGAGATACCTTTTTCGATCTGACCGTAAGCGCCAGTCATT
O09ig59     PCA assembly of {?OprF AtD?} (B09ig57)
GTATCTCTGACTCTACCTCTGTTGGTCTGATGCTGGGTGGTAACAACAACAA
O09ig60     PCA assembly of {?OprF AtD?} (B09ig57)
TACCTTTCAGGTCAGAGGTAGCGATGTCAACTTTGTTGTTGTTACCACCCAG
O09ig61     PCA assembly of {?OprF AtD?} (B09ig57)
TACCTCTGACCTGAAAGGTAACTCTGCTTACCTGGGTGGTTACGTTAAACAC
O09ig62     PCA assembly of {?OprF AtD?} (B09ig57)
ATACCAGCGATACCACGGAAAGCACCTTTGTCGTGTTTAACGTAACCACCCA
O09ig63     PCA assembly of {?OprF AtD?} (B09ig57)
TTCCGTGGTATCGCTGGTATCGGTATCCAGTACTCTGACTGGGACTCTCACC
O09ig64     PCA assembly of {?OprF AtD?} (B09ig57)
GTTTTCTTTGTAAGAGTCAGACATGGTGTTACGGTGAGAGTCCCAGTCAGAG
O09ig65     PCA assembly of {?OprF AtD?} (B09ig57)
CTGACTCTTACAAAGAAAACTACTCTGACCGTGGTCTGAACATCTACGCAGA
O09ig66     PCA assembly of {?OprF AtD?} (B09ig57)
GACCTTCGGTCAGTTCTTTGGTGTATTTACCCTCTGCGTAGATGTTCAGACC
O09ig67     PCA assembly of {?OprF AtD?} (B09ig57)
CAAAGAACTGACCGAAGGTCTGTTCCTGGAACCGAAAGTTGGTGTTTCTTAC
O09ig68     PCA assembly of {?OprF AtD?} (B09ig57)
GATTCGTTGATAGATTCCTGTTTGATGTAGTCGTAAGAAACACCAACTTTCG
O09ig69     PCA assembly of {?OprF AtD?} (B09ig57)
CAGGAATCTATCAACGAATCTAAAGACAAAGCTCTGGCTCTGGAAGTTGACG
O09ig70     PCA assembly of {?OprF AtD?} (B09ig57)
AACGTTACCAGACAGGGTGTCGAAGTCTTTACCGTCAACTTCCAGAGCCAGA
O09ig71     PCA assembly of {?OprF AtD?} (B09ig57)
ACACCCTGTCTGGTAACGTTGGTCTGGACCTGCGTAAAGAAATCGTTACCGA
O09ig72     PCA assembly of {?OprF AtD?} (B09ig57)
TAACACCAGCGGTCAGGTTGTGTTTACCTTTTTCGGTAACGATTTCTTTACG
O09ig73     PCA assembly of {?OprF AtD?} (B09ig57)
CAACCTGACCGCTGGTGTTAACTACACCCGTATCCTGTCTGGTGCTGACGAA
O09ig74     PCA assembly of {?OprF AtD?} (B09ig57)
GAGTTACCACCGAAGTTGCCAGTCAGGTTGTCTTCGTCAGCACCAGACAGGA
O09ig75     PCA assembly of {?OprF AtD?} (B09ig57)
GGCAACTTCGGTGGTAACTCTTTCGATATTCTGGTTCCGCAGAAAACCAAAG
O09ig76     PCA assembly of {?OprF AtD?} (B09ig57)
GATGTCGTATTTGATACCAACAGAAACGTTGTCTTTGGTTTTCTGCGGAACC
O09ig77     PCA assembly of {?OprF AtD?} (B09ig57)
TTGGTATCAAATACGACATCGAACTGGAAACTGGCGTTATGCTGGGTGCTAA
O09ig78     PCA assembly of {?OprF AtD?} (B09ig57)
CGTTAGGATCCGTAAGAAACTTTAGCACCCAGCATAACGCC

References