Difference between revisions of "Part:BBa K5078002"

Latest revision as of 00:18, 2 October 2024

PTC amiRNA

An aritificial micro RNA used to silence the expression of the Phosphate Transport Channel 1 (PTC1) gene in Chlamydomonas reinhardtii, thus removing the PTC1 transport protein from the central vacuole of the cell and preventing leakage of phosphate back into the environment in which our modified C. reinhardtii cell is placed.

Figure 1. Ptc amiRNA in a level 0 plasmid for later golden gate assembly.

Assembly verification

Successful insertion of the Ptc amiRNA was confirmed by PCR of the colonies, showing an increase of 100 bp of the PCR product.

Figure 2. PCR verification of cloning of Ptc amiRNA.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 112
Illegal PstI site found at 205
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 85
Illegal PstI site found at 112
Illegal PstI site found at 205
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 112
Illegal PstI site found at 205
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 112
Illegal PstI site found at 205
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K5078002 short</partinfo>
-An aritificial micro RNA used to silence the expression of the Phosphate Transport Channel 1 (PTC1) gene in Chlamydomonas reinhardtii thus removing the PTC1 transport protein from the central vacuole of the cell and preventing leakage of phosphate back into the environment in which our modified C. reinhardtii cell is placed.
+An aritificial micro RNA used to silence the expression of the Phosphate Transport Channel 1 (PTC1) gene in Chlamydomonas reinhardtii, thus removing the PTC1 transport protein from the central vacuole of the cell and preventing leakage of phosphate back into the environment in which our modified C. reinhardtii cell is placed.
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