Difference between revisions of "Part:BBa K197021:Design"
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<partinfo>BBa_K197021 short</partinfo> | <partinfo>BBa_K197021 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at: | |
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| + | [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page] | ||
===Source=== | ===Source=== | ||
Latest revision as of 05:10, 22 October 2009
EILD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
Source
Wobble Osc0028F/Osc0029R (136bp, EcoRI/BamHI) Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, 3171+910, L) Product is pBca9495AK-M10053 {<EILD>}
Osc0028F Forward construction of Leucine zipper EILD TCCGGgaattcATGagatctGTTAAAGAACTGGAAGACAAAAACGAAGAACTGCTGTCTGAAATCTACCACCTGGACAACGAAG Osc0029R Reverse construction of Leucine zipper EILD CTGGTggatccGCAACCACCACGTTCACCAACCAGTTTTTTCAGACGAGCAACTTCGTTGTCCAGGTGGTAG