Difference between revisions of "Part:BBa K5117007:Design"
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
removal of restriction sites which are used for RFC[10] or TypeIIS standards,  
+
removal of restriction sites which are used for RFC[10] or Type IIS standards,  
  
 
exchange of start codon (GTG > ATG) for usage in <i>Bacillus subtilis</i> as host organism
 
exchange of start codon (GTG > ATG) for usage in <i>Bacillus subtilis</i> as host organism

Latest revision as of 23:53, 1 October 2024


BhBglA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 564
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

removal of restriction sites which are used for RFC[10] or Type IIS standards,

exchange of start codon (GTG > ATG) for usage in Bacillus subtilis as host organism

Source

Bacillus halodurans