Difference between revisions of "Part:BBa K5439003:Design"

 
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With the DNA fragments purified from an agarose gel, we performed ligation at a molar ratio of 1:5 for vector and insert, as shown in <b>Figure 3</b>. The total vector concentration was 100 nanograms, whereas the reaction volume was 20 µL. Next, <b>Table 2</b> displays the protocol followed for the reaction.
  
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{| class="wikitable" style="margin:auto; text-align:center; length: 80%"
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|+ Table 2. DNA ligation conditions.
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|-
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!Reactive !! Quantity
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|-
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| style="text-align:center;" style="width: 80%;" | T4 DNA Ligase Buffer (10X) || 2 µL
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|-
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| style="text-align:center;" style="width: 80%;" | Vector DNA || 100 ng
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|--
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| style="text-align:center;" style="width: 80%;" | Insert DNA || 773.5 ng
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|-
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| style="text-align:center;" style="width: 80%;" | Nuclease-free water || To 20 µL
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|-
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| style="text-align:center;" style="width: 80%;" | T4 DNA Ligase || 1.5 µL
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|}

Revision as of 23:07, 1 October 2024

With the DNA fragments purified from an agarose gel, we performed ligation at a molar ratio of 1:5 for vector and insert, as shown in Figure 3. The total vector concentration was 100 nanograms, whereas the reaction volume was 20 µL. Next, Table 2 displays the protocol followed for the reaction.

Table 2. DNA ligation conditions.
Reactive Quantity
T4 DNA Ligase Buffer (10X) 2 µL
Vector DNA 100 ng
Insert DNA 773.5 ng
Nuclease-free water To 20 µL
T4 DNA Ligase 1.5 µL