Difference between revisions of "Part:BBa K5127009:Design"

 
Line 12: Line 12:
  
 
===Source===
 
===Source===
−
 
 
N/A
 
N/A
 +
 +
  
 
===References===
 
===References===
 +
Abramson, J., Adler, J., Dunger, J., Evans, R., Green, T., Pritzel, A., Ronneberger, O., Willmore, L., Ballard, A. J., Bambrick, J., Bodenstein, S. W., Evans, D. A., Hung, C. C., O'Neill, M., Reiman, D., Tunyasuvunakool, K., Wu, Z., Žemgulytė, A., Arvaniti, E., ... Jumper, J. M. (2024). Accurate structure prediction of biomolecular interactions with AlphaFold 3. Nature, 630(493-500). https://doi.org/10.1038/s41586-024-07487-w
 +
 +
Beabout, K., Ehrenworth Breedon, A. M., Blum, S. M., Miklos, A. E., Lux, M. W., Chávez, J. L., & Goodson, M. S. (2022). Detection of Bile Acids in Complex Matrices Using a Transcription Factor-Based Biosensor. ACS Biomaterials Science & Engineering, 9(9), 5151–5162. https://doi.org/10.1021/acsbiomaterials.2c01006

Revision as of 23:06, 1 October 2024


Brer-pBrer-gfp


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 400
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 653
    Illegal NheI site found at 676
    Illegal PstI site found at 400
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 400
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 400
    Illegal AgeI site found at 853
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.


Source

N/A


References

Abramson, J., Adler, J., Dunger, J., Evans, R., Green, T., Pritzel, A., Ronneberger, O., Willmore, L., Ballard, A. J., Bambrick, J., Bodenstein, S. W., Evans, D. A., Hung, C. C., O'Neill, M., Reiman, D., Tunyasuvunakool, K., Wu, Z., Žemgulytė, A., Arvaniti, E., ... Jumper, J. M. (2024). Accurate structure prediction of biomolecular interactions with AlphaFold 3. Nature, 630(493-500). https://doi.org/10.1038/s41586-024-07487-w

Beabout, K., Ehrenworth Breedon, A. M., Blum, S. M., Miklos, A. E., Lux, M. W., Chávez, J. L., & Goodson, M. S. (2022). Detection of Bile Acids in Complex Matrices Using a Transcription Factor-Based Biosensor. ACS Biomaterials Science & Engineering, 9(9), 5151–5162. https://doi.org/10.1021/acsbiomaterials.2c01006