Difference between revisions of "Part:BBa K5088675"
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K5088675 short</partinfo>
+
<partinfo>BBa_K5088675 short</partinfo>
 
__TOC__
 
__TOC__
 
<html>
 
<html>
Line 13: Line 13:
 
==Background==
 
==Background==
 
<html>
 
<html>
−
the expression system used the GFP from the plant collection in the iGEM distribution kit. The expression was driven by the 35S CaMV promoter, TMV 5'UTR, and 35S 3'UTR. This system is widely used for constitutive overexpression of transgenes in various plant species, including model organisms and economically important crops (<a href="https://doi.org/10.1016/j.cpb.2020.100179" target=blank_>1</a>).
+
For initial testing of our measurement system we used the <a href="https://parts.igem.org/Part:BBa_J428076" target=blank_>GFP from the plant collection in the iGEM distribution kit</a>. The expression was driven by the 35S CaMV promoter, TMV 5'UTR, and 35S 3'UTR. This system is widely used for constitutive overexpression of transgenes in various plant species, including model organisms and economically important crops (<a href="https://doi.org/10.1016/j.cpb.2020.100179" target=blank_>1</a>).
−
 
+
</html>
  
  
Line 20: Line 20:
  
 
==Results==
 
==Results==
−
 
+
<html>
 +
<center>
 
<figure>
 
<figure>
 
         <a href="https://static.igem.wiki/teams/5088/wiki/images/engineering/pgfp-sbol.png" target="_blank">
 
         <a href="https://static.igem.wiki/teams/5088/wiki/images/engineering/pgfp-sbol.png" target="_blank">
−
             <img src="https://static.igem.wiki/teams/5088/wiki/images/engineering/pgfp-sbol.png" width="900px" max-height:400px>
+
             <img src="https://static.igem.wiki/teams/5088/wiki/images/engineering/pgfp-sbol.png" width=500px>
 
         </a>
 
         </a>
 
         <figcaption><p><b>Figure 1:</b> SBOL scheme of <a href="https://parts.igem.org/Part:BBa_K5088675" target=blank_>Tarakate - Test construct GFP [BBa_K5088675]</a>.</figcaption>
 
         <figcaption><p><b>Figure 1:</b> SBOL scheme of <a href="https://parts.igem.org/Part:BBa_K5088675" target=blank_>Tarakate - Test construct GFP [BBa_K5088675]</a>.</figcaption>
 
</figure>
 
</figure>
 +
</center>
 +
 +
<center>
 +
<figure>
 +
        <a href="https://static.igem.wiki/teams/5088/wiki/images/engineering/nb-pgfp.png" target="_blank">
 +
            <img src="https://static.igem.wiki/teams/5088/wiki/images/engineering/nb-pgfp.png" width=500px>
 +
        </a>
 +
        <figcaption><p><b>Figure 2</b>: Successful expression of avGFP in N. benthamiana leaf disk assay using <a href="https://parts.igem.org/Part:BBa_K5088675" target=blank_>Tarakate - Test construct GFP [BBa_K5088675]</a>.</figcaption>
 +
</figure>
 +
</center>
 +
 +
 +
<center>
 +
<figure>
 +
        <a href="https://static.igem.wiki/teams/5088/wiki/images/engineering/protoplasts-gfp-pgfp.webp" target="_blank">
 +
            <img src="https://static.igem.wiki/teams/5088/wiki/images/engineering/protoplasts-gfp-pgfp.webp" width=500px>
 +
        </a>
 +
        <figcaption><p><b>Figure 3</b>: Successful expression of avGFP in protoplasts of Taraxacum officinale using <a href="https://parts.igem.org/Part:BBa_K5088675" target=blank_>Tarakate - Test construct GFP [BBa_K5088675]</a>.</figcaption>
 +
</figure>
 +
</center>
 +
 +
<center>
 +
<figure>
 +
        <a href="https://static.igem.wiki/teams/5088/wiki/images/engineering/protoplasts-gfp-pgfp.webp" target="_blank">
 +
            <img src="https://static.igem.wiki/teams/5088/wiki/images/engineering/protoplasts-gfp-pgfp.webp" width=500px>
 +
        </a>
 +
        <figcaption><p><b>Figure 4</b>: Successful expression of avGFP in roots of T. kok-saghyz using the CDB protocol and <a href="https://parts.igem.org/Part:BBa_K5088675" target=blank_>Tarakate - Test construct GFP [BBa_K5088675]</a>. A) Brightfield view of a transformed root of T. kok-saghyz B) The same root imaged under UV light.</figcaption>
 +
</figure>
 +
</center>
 +
 +
 +
 +
 +
</html>

Revision as of 21:47, 1 October 2024

Tarakate - Test construct GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 230
    Illegal XhoI site found at 1257
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

For initial testing of our measurement system we used the GFP from the plant collection in the iGEM distribution kit. The expression was driven by the 35S CaMV promoter, TMV 5'UTR, and 35S 3'UTR. This system is widely used for constitutive overexpression of transgenes in various plant species, including model organisms and economically important crops (1).



Results

Figure 1: SBOL scheme of Tarakate - Test construct GFP [BBa_K5088675].

Figure 2: Successful expression of avGFP in N. benthamiana leaf disk assay using Tarakate - Test construct GFP [BBa_K5088675].

Figure 3: Successful expression of avGFP in protoplasts of Taraxacum officinale using Tarakate - Test construct GFP [BBa_K5088675].

Figure 4: Successful expression of avGFP in roots of T. kok-saghyz using the CDB protocol and Tarakate - Test construct GFP [BBa_K5088675]. A) Brightfield view of a transformed root of T. kok-saghyz B) The same root imaged under UV light.