Difference between revisions of "Part:BBa K197030:Design"

(New page: __NOTOC__ <partinfo>GFP-LVA</partinfo> <partinfo>BBa_K197030 SequenceAndFeatures</partinfo> ===Design Notes=== ===Source=== <pre> PCR mv022/mv014 on pBca1256-K112109 (1492bp, E...)
 
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===Source===
 
===Source===
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synthesis
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<pre>
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PCR mv022/mv014 on pBca1256-K112109    (1492bp, EcoRI/BamHI/DpnI)
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
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Product is pBca1256-K112111
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-----------------------------------------------
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mv022 Forward Biobricking of <ligase!
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ccataGAATTCatgAGATCTattcttaaaattctgaacg
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mv014 Reverse Biobricking of ligase!
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cgttaGGATCCtcatagaccagttacctcatg
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</pre>
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===References===
 
===References===

Revision as of 04:23, 22 October 2009

No part name specified with partinfo tag.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 716
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 716
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 716
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 716
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 641


Design Notes

Source

synthesis

References