Difference between revisions of "Part:BBa K5492721"
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===<span class='h3bb'>Sequence and Features</span>=== | ===<span class='h3bb'>Sequence and Features</span>=== | ||
https://static.igem.wiki/teams/5492/registry/aptamer-fluorophore.jpg | https://static.igem.wiki/teams/5492/registry/aptamer-fluorophore.jpg | ||
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| + | Proposed Folding: | ||
| + | https://static.igem.wiki/teams/5492/registry/mfold/j1-fold.png | ||
<partinfo>BBa_K5492721 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5492721 SequenceAndFeatures</partinfo> | ||
Revision as of 21:20, 1 October 2024
J1_aptamer_with_fluorophore
J1 ssDNA aptamer sequence designed to specifically bind to histamine. The sequence contains a fluorophore molecule at its 5' end (5' Cy5™). j1_reverse_compliment_strand is complementary to this sequence, which contains a quencher molecule (3'Iowa Black® RQ) on its 3' end, which absorbs the light emitted by fluorophore until the aptamer binds histamine.
Usage and Biology
Aptamers are generally artificial ssDNA, RNA, or peptide oligomers which bind to specific target molecules. All ssDNA aptamers we utilise are proven to be able to bind specifically to histamine, thus preventing the binding of the molecule to a histamine receptor.
Sequence and Features
Proposed Folding:
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]