Difference between revisions of "Part:BBa K5286003"

 
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This part contains Hypsibius dujardini's heat soluble protein CAHS94063 coding sequence, and a 10XHisTag with a single stop codon for protein purification according to the protocol by Boothby et al.(2017):
  
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"Cells were then lysed by heat shock at 95°C for 15 min. Lysates were cooled at room temperature for 30 min. Insoluble components were removed by centrifugation at 20,000g and 10°C for 30 min."
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The protein can then be further purified with a HisTag purification kit.

Revision as of 21:11, 1 October 2024

This part contains Hypsibius dujardini's heat soluble protein CAHS94063 coding sequence, and a 10XHisTag with a single stop codon for protein purification according to the protocol by Boothby et al.(2017):

"Cells were then lysed by heat shock at 95°C for 15 min. Lysates were cooled at room temperature for 30 min. Insoluble components were removed by centrifugation at 20,000g and 10°C for 30 min."

The protein can then be further purified with a HisTag purification kit.