Difference between revisions of "Part:BBa K5286003"
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+ | This part contains Hypsibius dujardini's heat soluble protein CAHS94063 coding sequence, and a 10XHisTag with a single stop codon for protein purification according to the protocol by Boothby et al.(2017): | ||
+ | "Cells were then lysed by heat shock at 95°C for 15 min. Lysates were cooled at room temperature for 30 min. Insoluble components were removed by centrifugation at 20,000g and 10°C for 30 min." | ||
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+ | The protein can then be further purified with a HisTag purification kit. |
Revision as of 21:11, 1 October 2024
This part contains Hypsibius dujardini's heat soluble protein CAHS94063 coding sequence, and a 10XHisTag with a single stop codon for protein purification according to the protocol by Boothby et al.(2017):
"Cells were then lysed by heat shock at 95°C for 15 min. Lysates were cooled at room temperature for 30 min. Insoluble components were removed by centrifugation at 20,000g and 10°C for 30 min."
The protein can then be further purified with a HisTag purification kit.